association between the enterotoxin production and presence of coa, nuc genes among staphylococcus aureus isolated from various sources, in shiraz

Authors

r. moghassem hamidi

s. hosseinzadeh

s. s. shekarforoush

m. poormontaseri

a. derakhshandeh

abstract

the present study was aimed to identify the frequency of coagulase (coa) and thermonuclease (nuc) genes and staphylococcal enterotoxin a (sea) production among staphylococcus aureus isolated from various sources in shiraz. moreover, the correlation between the sea gene and coagulase and thermonuclease enzymes is also considered. a total of 100 s. aureus were isolated from various sources including 40 humans, 30 animals and 30 food samples by the routine biochemical tests. the frequency of coa, nucand sea genes was evaluated by pcr assay. correlation among those genes was finally evaluated by statistical analysis. the pcr results showed that the prevalence of coa, nuc and sea genes was 91%, 100% and 14%, respectively. the evaluation of the enterotoxin production indicated that 78.6% of the sea gene was expressed. the presence of enterotoxin a was not necessarily correlated to the production of toxin. as a final conclusion to detect the enterotoxigenic strains, both genotypic and phenotypic methods are highly recommended.

Upgrade to premium to download articles

Sign up to access the full text

Already have an account?login

similar resources

Association between the enterotoxin production and presence of Coa, Nuc genes among Staphylococcus aureus isolated from various sources, in Shiraz

The present study was aimed to identify the frequency of coagulase (Coa) and thermonuclease (Nuc) genes and Staphylococcal enterotoxin A (Sea) production among Staphylococcus aureus isolated from various sources in Shiraz. Moreover, the correlation between the Sea gene and coagulase and thermonuclease enzymes is also considered. A total of 100 S. aureus were isolated from various sources includ...

full text

Association between the enterotoxin production and presence of Coa, Nuc genes among Staphylococcus aureus isolated from various sources, in Shiraz.

The present study was aimed to identify the frequency of coagulase (Coa) and thermonuclease (Nuc) genes and Staphylococcal enterotoxin A (Sea) production among Staphylococcus aureus isolated from various sources in Shiraz. Moreover, the correlation between the Sea gene and coagulase and thermonuclease enzymes is also considered. A total of 100 S. aureus were isolated from various sources includ...

full text

Enterotoxin gene profiles among Staphylococcus aureus isolated from raw milk

Milk is considered a nutritious food because it contains several important nutrients including proteins and vitamins. Conversely, it can be a vehicle for several pathogenic bacteria such as Staphylococcus aureus. This study aimed to analyze the frequency of genes encoding the nine Staphylococcal enterotoxins (SEs) and enterotoxin gene profiles in S. aureus isolates derived from raw bovine milk....

full text

Identification of the classical enterotoxin genes of Staphylococcus aureus in various foods by multiplex PCR assay

Background: An annual update of information about the prevalence of Staphylococcus aureus and staphylococcal enterotoxins (SEs) genes is required in every geographic area. Aims: This study was conducted to investigate the prevalence of the bacterium and type of associated enterotoxin genes in different food samples, using multiplex polymerase ...

full text

Detection of Classic Enterotoxin Genes and Coagulase Gene Typing of Staphylococcus Aureus Isolated from Raw Cow Milk in Isfahan

Background and Aims: Staphylococcus aureus (S. aureus) is considered as one of the most dangerous pathogenic bacteria due to the production of extracellular toxins. The objective of this study was to determine the prevalence of S. aureus and to characterize the recovered strains for their enterotoxin-producing genes in raw cow milk. Materials and Methods: During 9 months duration of the s...

full text

Enterotoxin gene profiles among Staphylococcus aureus isolated from raw milk.

Milk is considered a nutritious food because it contains several important nutrients including proteins and vitamins. Conversely, it can be a vehicle for several pathogenic bacteria such as Staphylococcus aureus. This study aimed to analyze the frequency of genes encoding the nine Staphylococcal enterotoxins (SEs) and enterotoxin gene profiles in S. aureus isolates derived from raw bovine milk....

full text

My Resources

Save resource for easier access later


Journal title:
iranian journal of veterinary research

Publisher: shiraz university

ISSN 1728-1997

volume 16

issue 4 2015

Hosted on Doprax cloud platform doprax.com

copyright © 2015-2023